Transcriptional stimulation by CaPO4-DNA precipitates.
نویسندگان
چکیده
Genes in human chromosomes that normally require induction by alpha-interferon are activated after calcium phosphate (CaPO4) transfection, but not after DEAE-dextran transfection. The c-fos gene and genes stimulated by gamma-interferon also are affected by CaPO4-DNA precipitates, but the calcium ionophore A23187 stimulates only c-fos among this group. These results suggest caution not only in choosing gene transfer methods, but also in interpreting experiments aimed at understanding the role of second messengers in gene activation.
منابع مشابه
Production of high titre helper-free recombinant retroviral vectors by lipofection.
High titre stocks of replication-defective retroviral vectors are generally prepared by stable introduction of cloned proviraJ DNA into packaging cell lines that supply in-trans all the proteins necessary for viral assembly (1). However, different vectors are packaged with different efficiencies and the expression of transgenes and activity of heterologous internal promoter elements may vary wi...
متن کاملTransfection maximizer increases the efficiency of calcium phosphate transfections with mammalian cells.
A variety of methods have been described for introducing DNA into cultured mammalian cells. Among these, calcium phosphate (CaPO4)-mediated transfection has gained wide acceptance due to the procedure's low cost, simplicity and suitability for a wide range of cell types. However, this method is limited by relatively low transfection efficiencies and poor reproducibility. To improve the utility ...
متن کاملTruncated active matrix metalloproteinase-8 gene expression in HepG2 cells is active against native type I collagen.
BACKGROUND/AIMS Excess type I collagen accumulation is a major feature of fibrotic diseases such as liver cirrhosis. Reversion of this disease has not been fully accomplished. Physiologically, collagen is degraded by interstitial collagenases, neutrophil collagenase (MMP-8) being the most active against type I collagen. Introduction of MMP-8 gene into liver cells could be an advantageous tool t...
متن کاملTranscriptional effects of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter through the estrogen receptor a in MDA-MB 231 breast cancer cell line
BACKGROUND: Some of metal ions as environmental pollutants show estrogenic activity. This xenostrogenic compounds can be caused carcinogenicity in organs. The mechanism of carcinogenicity of metal ions is not clarified. OBJECTIVES: In this study, we investigated the Transcriptional effects of variety of metal ions on the bovine oxytocin and the thymidine kinase-ERE promoter by estrogen receptor...
متن کاملHyperglycemia Suppresses Calcium Phosphate–Induced Aneurysm Formation Through Inhibition of Macrophage Activation
BACKGROUND The aim of this study was to elucidate aspects of diabetes mellitus-induced suppression of aneurysm. We hypothesized that high glucose suppresses aneurysm by inhibiting macrophage activation via activation of Nr1h2 (also known as liver X receptor β), recently characterized as a glucose-sensing nuclear receptor. METHODS AND RESULTS Calcium phosphate (CaPO4)-induced aneurysm formatio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Nucleic acids research
دوره 16 4 شماره
صفحات -
تاریخ انتشار 1988